Annotine is a lycopodane-type alkaloid isolated from the Icelandic dub moss Lycopodium annotinum ssp.
Annotine is a lycopodium alkaloid with a lycopodane-type skeleton (Fig.
We, therefore, used DCs and co-cultures of DCs and T cells to study the potential of annotine to affect DC activation and how annotine-treated DCs modulate T cell responses.
Annotine was isolated from the Icelandic club moss Lycopodium annotinum ssp.
4] cells/well DCs previously matured in the absence (C-mDCs) or presence (A-mDCs) of annotine at 100 [micro]g/ml.
Results are presented as secretion index (SI), which is the concentration of the cytokine in culture medium from cells stimulated in the presence of annotine divided by the concentration of the cytokine in culture medium from cells stimulated in the absence of annotine.
Annotine at 100 [micro]g/ml did not affect viability of mDCs as assessed by trypan blue staining and counting live cells (data not shown).
Annotine did not affect expression of surface molecules on mDCs cultured alone
Maturing DCs in the presence of annotine (100 [micro]g/ml) did not affect their expression levels (MFI) or the percentage of cells expressing CD 14, CD86 or HLA-DR (data not shown).
Annotine decreased mDC secretion of IL-6, IL-10, IL-12p40 and IL-23 but increased the ratio ofIL-12p40/lL-10
DCs matured in the presence of the highest concentration of annotine had a tendency toward secreting less IL-12p40 (P = 0.
DCs matured in the presence of annotine secreted less of the proinflammatory cytokines 1L-6 (Fig.