2008) with Artemia salina
as the model organism to investigate the cytotoxicity of the crude methanolic extract of S.
Key words: cytotoxic, brine shrimp, Artemia salina
, extract, plant, seed, Crateva religiosa, Diospyros dichrophylla, Olax subscorpioidea
McLaughlin (1991) has reported that the results obtained with Artemia salina
(brine shrimp) are quantitative and reproducible, and the activities parallel cytotoxicities.
Our aim in the present work was to screen medicinal plants with different known therapeutic activities for their cytotoxic effect on Artemia salina
and correlate toxicity results with their known ethnopharmacological activities.
5g Artemia salina
were transferred into artificial sea water and incubated for 24 hours under room temperature with oxygen supplement and light intensity.
The taxonomic study of Artemia populations in the Western Old World, including Italy, the South of France and the Iberian Peninsula (Spain and Portugal), together with North Africa, is interesting owing to the presence of the Mediterranean bisexual Artemia salina
Brine shrimp eggs, Artemia salina
leach were hatched in artificial seawater prepared by dissolving 38g of sea salt in 1L of distilled water.
The toxicity assay using Artemia salina
was carried out as described by Aboaba et al (16).
UPTAKE AND TOXICITY PATTERNS OF ZINC OXIDE NANOPARTICLES ON ARTEMIA SALINA
To determine the toxic effects on Artemia salina
(brine shrimp) the method of Meyer et al  was adopted.
Two separate sets of laboratory experiments were conducted to determine the density-dependent predation rates of juvenile Litopenaeus setiferus feeding directly on (1) zooplanktonic prey Artemia salina
in the water column and (2) benthic prey Mercenaria mercenaria in sediment.
A microwell cytotoxicity assay using Artemia salina