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autoimmunity |
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Methods: Immortalized human salivary acinar cell line NS-SV-AC cells were incubated with EGCG (epigallocat-echin-3 gallate), the most abundant GTP, prior to determination of expression changes in major autoantigens by microarray, RT-PCR and Western analysis. The peptide, now called MBP8298, targets a particular stretch of amino acids of human myelin basic protein (MBP), a major structural protein of myelin that is one of the candidate autoantigens in MS. The reacting IgG class antibody, which was purified from normal human serum by affinity chromatography on bupleuran 2IIc (a pharmacologically active pectic polysaccharide from the roots of Bupleurum falcatum)-immobilized Sepharose, showed cross-reactivity not only with some other pharmacologically active pectic polysaccharides from other medicinal herbs but also with autoantigens such as single-strand DNA, myosin and tublin from mammals. |
Autoantigens |
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