After RNA hybridization, tag-conjugating Cy3
dye was circulated through the microfluidic chip for dye staining.
Attenuator 5x reflector turret, motorized Fluorescence filters GFP (EX BP 470/40, FT 495, EM BP 525/50) Fluorescence filter Cy3
(EX BP 550/25, FT 570, EM BP 605/70) 4x lens revolver, coded, lens lift = 22 mm Mechanical parfocal adjustment 10x diving lens, apochromatically corrected VIS - IR; NA = 0.
The samples were randomly labeled with Cy3
Researchers in Japan have recently improved the existing probing techniques using a cyanine dye called Cy3
Nuclei were stained for 10 min in the dark with DAPI and cells were observed under a fluorescence inverted microscope using filter set for Cy3
Analogues of ATP modified with Cy3
or BODIPY fluorescent dyes, which shift excitation bands into the visible range to reduce phototoxicity, are also available.
, Cy5, Deep Purple, pH 3-10 NL IPG strip (24cm),1,4-dithiothreitol (DTT), Acrylamide (Acr), carrier ampholyte, lysine, sodium dodecyl sulfate (SDS), N,N'-methylenebisacrylamide (Bis), 2D-Quant protein assay kit, thiourea and urea were obtained from GE Healthcare (US).
Cells were then incubated with Cy5 anti-rabbit and Cy3
anti-mouse (both 1:400) secondary antibodies for 1 hour at room temperature.
Al dia siguiente, las muestras se lavaron 4 veces con PBS 1X y se bloqueo con albumina al 1% durante 15 min, despues se retiro la albumina, se coloco el anticuerpo secundario Cy3
anti-conejo, dejando reposar en oscuridad durante 1 h a temperatura ambiente.
1x SSC at 40C after hybridization then biotin and digoxygenin were detected through FITC conjugated anti-digoxygenin antibodies (Roche Germany) and streptavidin Cy3
2002), the probes used for hybridization were labeled with Cy3
and FAM (Fluorescein) purchased at Microsynth(r) (Switzerland).
Se enjuago con PBS y se incubo con anticuerpos secundarios antiraton conjugados con FITC (fluorescencia isotiocianato, 1:100) para NR1 y anti-conejo conjugado con Cy3
(1:200) para SV2.