also reported that Chkl dependence of DATS-induced mitotic arrest in human cancer cells is not influenced by the p53 status and cells arrested in mitosis upon DATS exposure are driven to apoptotic DNA fragmentation (Xiao et al.
Furthermore, oral treatment of DATS inhibited incidence and burden of poorly differentiated carcinoma and pulmonary metastasis multiplicity in a transgenic mouse model of prostate cancer without causing any harmful side effects (Singh et al.
Assessment of cell morphology and viability ofCT26 cells after exposed to DATS
One group is lipid-soluble, such as diallyl sulfide (DAS), DADS and DATS, and the other group is water-soluble compounds [gamma]-glutamyl S-allylcysteine group such as S-allylcysteine (SAC) and S-allylmercaptocysteine (SAMC) (Herman-Antosiewicz and Singh 2004; Thomson and Ali 2003).
In this study, we demonstrated that garlic derivatives, DATS, suppressed proliferation of T24 bladder cancer cells lines by inducing apoptosis through P13K/Akt pathway, thus down-regulating anti-apoptotic protein Bcl-2 and Bcl-xL, up-regulating pro-apoptotic protein Bax, leading to the activation of caspase-3 and poly(ADP-ribose) polymerase (PARP).