The pellet was suspended in 100 [micro]L of DNase
and RNase-free water and 100 [micro]L of 2 x loading buffer were added for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
RNA was extracted using TRIzof reagent (Life Technologies) followed by DNase
I (Thermo Fisher Scientific, Waltham, MA, USA) digestion according to the manufacturer's protocols.
RNA treated with DNase
I was prepared from AcSINV-3-infected Sf21 cells revealing an increase in SINV-3 transcript production over 5 d (Fig.
This research shows that while we may not be able to target the bacteria in biofilms directly, scientists can take an indirect route by first removing the protective layer using enzymes, such as DNase
, and then subsequently targeting the Campylobacter bacteria that are inside the biofilm.
Characterization of a virulence-associated and cell-wall-located DNase
of Streptococcus pyogenes.
activity was determined in serum by a method of Kunitz  with some modification, where [0.
The protective effect of nanoparticles was detected using DNase
demonstrated that Usp is a protein having DNase
activity whose expression is toxic to E.
After three years of research, we found that the water quality, control of bacterial contaminations (sources of DNase
RNase and Protease) and a few other parameters are the key to make high quality SDS.
mannitol motility test, indole production test, urease test, citrate utilization, methyl red test, voges-proskauer's test, catalase test, oxidase test, nitrate reduction test, aminoacid utilization test, test for production of amylase, caseinase, dnase
, sugar utilization test, phenylalanine deaminase test, cellulase test, ONPG (P-galactosidase enzyme), gelatinase test, H2S production test, melonate test for characterization and identification of the isolated bacteria (7,8).
It has been shown that conversion of DNA into a DNase
resistant form occurs during ice incubation after heat shock step (1).
In the modified protocol, the final RNA pellet was washed with 75% ethanol and then air-dried before resuspension in a cocktail con taining 2 IU of DNase
I (Invitrogen), 1X DNase
I buffer, and 20 IU of SUPERase-In RNase Inhibitor (Ambion, Streetsville, Ontario, Canada).