2], 200 mM each deoxynucleotide
triphosphate (Amersham Pharmacia Biotech), 7.
In brief, DNA fragments of each gene were amplified in 50-[micro]L reaction mixture by using boiled bacterial suspension with 200 [micro]M of deoxynucleotide
triphosphate, 1 [micro]M of the primer pairs, Taq buffer (QIAGEN GmbH, Hilden, Germany), and 2.
2], 1x Reverse Transcription Buffer, 1 mmol/L deoxynucleotide
triphosphate mixture, 1 unit/[micro]L recombinant RNasin[R] ribonuclease inhibitor, 0.
Gaithersburg, MD), 250 mM (each) deoxynucleotide
triphosphate (GIBCO Invitrogen Corp.
Reaction conditions for the PPV assay were established for the Applied Biosystems 7700/7900/7500 Sequence Detection System series in a total reaction volume of 25 [micro]L containing 1 x PCR buffer, 5 mM MgClz, 1 mM deoxynucleotide
triphosphate mixture with dUTP, 1 [micro]M ROX, 1 U of Platinum Taq polymerase (Invitrogen), 7.
2 [micro]M for each primer, 200 [micro]M for each deoxynucleotide
triphosphate (Promega Corp.
1% Triton X-100), 1 [micro]M of each primer, 200 [micro]M deoxynucleotide
triphosphate, and 1 U Taq polymerase (Boehringer Mannheim, Germany) in a 25-[micro]L volume (14).
2 mM each of the deoxynucleotide
triphosphates, and 1 [micro]M each of the primers.
triphosphates were used to allow fluorescence detection of the extension products by use of a microarray system of spotted tag oligonucleotides complementary to signature tags on the extension primers.
The PCR reaction for G801A was carried out in a 25-[micro]L final volume with 80 nM of each oligonucleotide primer (sense, 5'-CAGTCAACCTGGGCAAAGCC-3'; antisense, 5'-AGCTTTGGTCCTGAGAGTCC-3'), 100 [micro]M of each deoxynucleotide
2], 200 [micro] M of each deoxynucleotide
triphosphate, 1 x PCR buffer, 1.
The PCR instruments market includes buffers and deoxynucleotide
triphosphates[dNTPs] (buffers, contamination control kits, dNTP mixes), controls and cDNAs, primers (real-time PCR primers, human linkage mapping sets, mouse mapping primers), PCR enzymes and kits (contamination control kits, general PCR, fast PCR, multiplex PCR, wax beads for hot start PCR, high-fidelity PCR kits and other kits), accessories, reaction plates, adhesive films and thermal cyclers.