Dereplication

De`rep`li`ca´tion


n.1.(Biochem.) the process of testing samples of mixtures which are active in a screening process, so as to recognize and eliminate from consideration those active substances already studied; - a stage subsequent to the preliminary screening in the process of discovery of new pharmacologically active substances in mixtures of natural products; - also called counterscreening. See screening.
References in periodicals archive ?
Methods: While exploring the bioactive secondary metabolites, a sensitive and reliable LC-MS based dereplication approach was applied to identify four compounds A-D from fungal extract.
UPLC-MS technique is very power full technique which help us in identifying compounds with satisfactory knowledge for efficient dereplication.
This project proposes to employ acombination of chemical fingerprinting-based dereplication, assay-guided fractionation and reversed-phase preparative highperformance chromatographic methods to identify potential anticancer and antimalarial lead compounds from plants of thegenera mentioned above, selected on the basis of ethnobotanical information as well as literature data.
ARM can uniformly distribute the requests of hotspot data to other storage nodes by performing active replication and efficiently utilize storage resources by executing on-demand dereplication.
Based on the microscopic and cultural characteristics, 54 different actinomycete isolates were selected after dereplication of 18 isolates.
The MALDI Biotyper family of systems provides molecular detection, as well as taxonomical classification or dereplication of microorganisms such as bacteria, yeasts and fungi, using proteomic fingerprinting by high-throughput MALDI-TOF mass spectrometry.
The process involved: bacterial culture miniaturization, solid phase extraction of the products using the resin Diaion[TM] HP-20, the analysis by UPLC-MS of the products and finally the generation of the library, that allows to discard culture media components detecting 46 compounds produced by all bacteria and the subsequent identification of 10 of them, using the database Antibase 2007[TM] as a tool for dereplication of these substances.
Dereplication of Streptomyces soil isolates and detection of specific biosynthetic genes using an automated ribotyping instrument.
Bruker's proprietary MALDI Biotyper solution enables molecular identification, taxonomical classification or dereplication of microorganisms like bacteria, yeasts and fungi.
With new case studies and illustrations, updated/new material on aspects of bioactive natural product research treat topics including chemical extraction, nuclear magnetic resonance (NMR)-based structure determination, liquid chromatography linked with mass spectrometry and NMR spectroscopy, dereplication approaches, and novel bioassay development.
Fungal metabolite screening: database of 474 mycotoxins and fungal metabolites for dereplication by standardised liquid chromatography-UV-mass spectrometry methodology.
The dedicated MALDI Biotyper solution enables molecular identification, and taxonomical classification or dereplication of microorganisms like bacteria, yeasts and fungi.