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DNA sequencing
(redirected from Dye terminator sequencing)

   Also found in: Encyclopedia, Wikipedia 0.01 sec.
DNA sequencing
The determination of the sequence of nucleotides in a sample of DNA.


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PCR products were purified by using the QIAquick gel extraction kit (Qiagen, Leusden, the Netherlands) and sequenced with the Big Dye Terminator sequencing kit, version 3.
The nucleotide sequence for each amplicon was determined by using Big Dye Terminator Sequencing Ready Reaction kits with AmpliTaq DNA polymerase FS, as described by the supplier (Applied Biosystems, Warrington, UK).
 
 
 
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