TRIpure Reagent was purchased from Beijing BIOTEKE Biotechnology Company (Beijing) and RevertAid[TM] First Strand cDNA Synthesis Kit was from FERMENTAS
Affymetrix Agilent Technologies Ambion Pierce Applied Biosystems BD Biosciences Beckman Coulter Bio-Rad Calbiochem Clontech Dako Stratagene Eppendorf Fermentas
GE Healthcare Invitrogen Waters Millipore New England Biolabs PerkinElmer Promega Qiagen R&D Systems Roche Applied Science Santa Cruz Biotechnology Sigma-Aldrich Takara Thermo Fisher Scientific VWR International
Contractor name : U DAROJI AKCINE BENDROVE FERMENTAS
Ligation of sodC amplicons into cloning vector pJET1--The purified sodC DNA amplicon was ligated into pJET1 using GeneJET PCR Cloning kit (MBI, Fermentas
Equal amounts of RNA (1 [micro]g) were reverse transcribed using a first-strand cDNAsynthesis kit (MBI Fermentas
, Vilnius, Lithuania).
The synthesized cDNA was amplified using the PCR reagent (Taq polymerase: MBI Fermentas
, lot: EP0402; d NTP Mix: MBI Fermentas
, USA: R0191).
The cells were rinsed in PBS and total RNA was isolated by guanidinium isothiocyanate using the TRIzol reagent (MBI Fermentas
, Lithuania) according to the manufacturer's instructions.
Of the ten biotech players in Lithuania, three of them (namely Fermentas
, Sicor and Biocentras) already operate on a global scale, indicating the country's strength in this area.
Viktoras Butkus, Chairman & CEO, Fermentas
Life Sciences, Lithuania: "Fermentas
has opened a new subsidiary in Shenzen.
The scientific vendors will be on hand from Noon until 2:00pm and some of the companies included are Advanced Microarray, Applied BioSystems, Applied Microarray, Bio-Rad, Biotage, Fermentas
, GE Healthcare, Laboratory Equipment Company, Molecular Devices, Rainin, Panomics and Zeiss.
For RFLP analysis, a 20-[micro]L aliquot containing 10 [micro]L of the PCR product was digested with 1 (T+30/in2G), 2 (+138/ ex1ins/delA), 5 (G+356/in4, T+834/ex5C, and T+881/ ex5C), 6 (G-1396A), or 10 U (G-46/in1A) of assay-specific restriction enzymes (MBI Fermentas
GmbH; see the online Data Supplement) at the enzyme-specific temperatures overnight.
5 [micro]L BsuRI endonuclease (MBI Fermentas
, Burlington, Ontario, Canada), and all digested products were analyzed by agarose gel electrophoresis (12).