For over 35 years, Fermentas
led the industry world-wide in the discovery, manufacturing and marketing of quality molecular biology products for life science research and diagnostics.
Gene expression analysis was carried out by mRNA extraction from liver tissue homogenate, using Vivantis GF-1 Total RNA Extraction Kit Cat# GF-TR-100, followed by cDNA synthesis by using the Fermentas
RevertAid First Strand cDNA Synthesis Kit #K1621.
1 mM IPTG (isopropyl-[beta]-d-thiogalacto-pyranoside), purchased from Fermentas
, EU, was applied to induce protein expression and cultivation was continued at 23[degrees]C with shaking (200 rpm) overnight.
Tenders are invited for Supply Of X-Glucurono Sodium Salt (5 Bromo-4-Chloro-3-Indolyl A-D-Glucuronide Sodium Salt) For Gus Detection, X-Gluc: Himedia Make, Terminal Deoxynucleotidyl Transferase (500units) Fermentas
Studied examples in biotechnology are Lithuanian enterprises Fermentas
and Sicor Biotech, as well as Quattromed from Estonia.
After denaturation of RNA at 70[degrees]C for 5 min, cDNA was synthesized by using Moloney murine leukemia virus RT and random hexamers (MBI Fermentas
, Vilnius, Lithuania), by incubating at 25[degrees]C for 10 min and thereafter at 37[degrees]C for 1 h.
En cada pozo se sirvieron 5 [micron]l del producto de PCR diluidos en 2 [micron]l de buffer carga 1X (fermentas
[R]) y se utilizaron 2 [micron]l de marcador de peso molecular (low range Fermentas
All PCR reactions were performed in 200 [micro]l transparent PCR tubes on a peltier-based thermal cycler (PTC100, MJ Research, USA) using reagents from Fermentas
Life Sciences (Fermentas
, Lithuania) in a total reaction volume of 50 [micro]l containing nearly 100 ng genomic DNA, 1.
2]) (MBI Fermentas
, Burlington, Canada), 3 [micro]l of 2 mM deoxyribonucleotide triphosphates (dNTPs) mixture (MBI Fermentas
, Burlington, Canada), 2.
2mM de una solucion de los cuatro deoxinu cleotidos trifosfatados (dNTPs; FERMENTAS
UAB, CANADA), 2.
THERMO FISHER WRAPS UP CANADIAN FERMENTAS
M2 EQUITYBITES-19 July 2010-Thermo Fisher acquires Fermentas
International Inc in Ontario(C)2010 M2 COMMUNICATIONS http://www.