RepliCel's unique manufacturing technology allows for isolation of fibroblasts
derived from anagen-hair follicle mesenchymal tissue, which elicit more efficient replication potential in culture.
These patients, usually treated with chemo-radiotherapy (CRT), often develop a CRT-induced fibrosis, which is characterized by an overgrowth of fibroblasts
at tumor-treated areas.
TNF-[alpha]-stimulated rheumatoid arthritis (RA) synovial fibroblasts
were used to elucidate the underlying mechanisms of action.
Cardiovascular scientists at Houston Methodist, with colleagues at Stanford University and Cincinnati Children's Hospital, learned that fibroblasts
-- cells that causes scarring and are plentiful throughout the human body -- can be coaxed into becoming endothelium, an entirely different type of adult cell that forms the lining of blood vessels.
133 CONVERTING PATIENT FIBROBLASTS
TO MOEL ASTROCYTE TOXICITY IN FAMILIAL AND SPORADIC ALS
are actively dividing cells and provide structural framework (stroma, cytoskeleton) for many tissues.
Fibronectin crosslinks with fibrin to create an adhesive scaffolding upon which the fibroblasts
As part of the chronic inflammatory reaction working through complex cytokine signaling interactions, activated fibroblasts
exit near-by vascularity, migrate to the area surrounding the implant, and work closely with macrophages in an effort to seal the implanted material away from the body within a layer of fibrous tissue.
To explore how the cells' surroundings might improve the efficiency of reprogramming, Yen Peng Kong, a post-doctoral researcher in the lab, attempted to turn scarring cells, or fibroblasts
, into heart muscle cells while growing them in gels of varying stiffness.
Then specialised repair cells such as the fibroblasts
multiply and migrate to the area that has been damaged.
showed that inhibition of fibroblast
activation protein and dipeptidylpeptidase increases cartilage invasion by rheumatoid arthritis synovial fibroblasts
Our findings here serve as a proof of concept that human fibroblasts
can be reprogrammed successfully into beating heart cells.