In Group C no intervention was done for first 4 days, on 5th day rabbits were given acute nephrotoxic single dose of amphotericin B deoxycholate6 (inj Fungizone
, Bristol Myers Squibb, Turkey), 4mg/kg as slow IV infusion diluted in 5% dextrose water and sacrificed on 6th day.
(Sigma-Aldrich, USA) and 1% gentamicin (Sigma-Aldrich).
A 450 [micro]L aliquot comprised of 400 [micro]L of Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS, 100 U/mL of penicillin and streptomycin, and 1 U/mL of fungizone
and gentamycin was added to each tube, and the sample was homogenized using a micropestle.
After the mosquitoes were sedated, the legs were removed from 12-30 mosquitoes and placed in 1 mL mosquito diluent (20% heat-inactivated fetal bovine serum in Dulbecco phosphate-buffered saline plus 50 [micro]g/mL penicillin/streptomycin, 50 [micro]g/mL gentamicin, and 2 [micro]g/mL Fungizone
[Sigma Aldrich, St.
5 mm trephine drill (Smith & Nephew, Hoofddorp, NL) while cooling with sterile phosphate-buffered saline (PBS; Sigma, Zwijndrecht, NL) supplemented with 200 U/ml penicillin (Lonza, Westburg, Leusden, NL), 200 [micro]g/ml streptomycin (Lonza, Westburg, Leusden, NL) and 5 [micro]g/ml fungizone
(LifeTechnologies, Bleiswijk, NL).
and fetal bovine serum (FBS) were obtained from Gibco (Bristol, UK), and trypsin-EDTA was obtained from Fluka (Gillingham, UK).
All collected cells were pooled as the hPDLs, and cultured in DMEM with 10% fetal calf serum, penicillin, streptomycin, and fungizone
for 1 week, whereas the tooth was placed in flask and cells slowly migrated from the root surfaces for 3 weeks were considered as hRDCs according to that described in the previous study.
3) Culture medium- The commonly used culture medium for oral mucosa reconstruction is Dulbecco's modified Eagle medium (DMEM)-Ham's F-12 medium (3:1), supplemented with fetal calf serum (FCS), glutamine, epidermal growth factor (EGF), hydrocortisone, adenine, insulin, transferrin, triiodothyronine, fungizone
, penicillin, and streptomycin (11).
(amphotericin B) (product monograph) [electronic version].
To activate and prepare virus for harvesting, kid kidney cells and testis were grown in tissue culture flasks at 37 Co in the growth medium containing 10% foetal calf serum, 1% fungizone
Confusion arose as the original "conventional" form, branded as Fungizone
, has a dose of about 1mg per kg of a patient's weight, but modern forms, branded as Ambizone and Abelcet, had dosages of 3mg and 5mg per kg.
The supernatant was collected and treated with antibiotic (penicillin 10,000 units/ml; streptomycin 10,000 units/ ml) and fungizone
(250 mg/ml) for 30-60 min at 4[degrees]C before inoculation into chicken embryos.