Gram's stain


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Gram stain

 (grăm) also Gram's stain (grămz)
n.
A staining technique used to classify bacteria in which a bacterial specimen is first stained with crystal violet, then treated with an iodine solution, decolorized with alcohol, and counterstained with safranin. Because of differences in cell wall structure, gram-positive bacteria retain the violet stain whereas gram-negative bacteria do not. Also called Gram's method.

[After Hans Christian Joachim Gram (1853-1938), Danish physician.]
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.Gram's stain - a staining technique used to classify bacteria; bacteria are stained with gentian violet and then treated with Gram's solution; after being decolorized with alcohol and treated with safranine and washed in water, those that retain the gentian violet are Gram-positive and those that do not retain it are Gram-negative
staining - (histology) the use of a dye to color specimens for microscopic study
References in periodicals archive ?
We compared polymerase chain reaction (PCR) of cloacal swab samples and fecal Gram's stain (FGS) for diagnosis of active shedding of M ornithogaster in a captive flock of budgerigars (Melopsitiacus undulatus).
The specimen was then transported to the bacteriology laboratory for Gram's stain and possible inoculation to the media.
Direct impression smears were prepared from the biopsy tissues on a clean glass slide and were stained with Gram's stain.
Surgical tissue Gram's stain and culture were nonrevealing, but fungal culture grew P.
As DNA tests become more accurate, fewer healthcare professionals are relying on the Gram's stain, which is reliable in only about half of all women, and culture testing, which can delay diagnosis by several days.
Sputum gram's stain in community-acquired pneumococcal pneumonia.
The aspirate was evaluated by Gram's stain, aerobic and anaerobic cultures, and Warthin-Starry silver staining (figure 3).
The subset of women who had intermediate-grade bacterial vaginosis at baseline according to Gram's stain scores were more likely than those with high-grade bacterial vaginosis to have lower cure rates if they took lactobacillus and did not become colonized.
They found that the subset of women who had intermediate-grade BV at baseline according to Gram's stain scores were more likely than those with high-grade BV to have lower cure rates if they took lactobacillus and did not become colonized.
Gram's stain remains one of the most valuable methods we have for identifying isolates accurately and rapidly.