Gram's stain

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Gram stain

 (grăm) also Gram's stain (grămz)
A staining technique used to classify bacteria in which a bacterial specimen is first stained with crystal violet, then treated with an iodine solution, decolorized with alcohol, and counterstained with safranin. Because of differences in cell wall structure, gram-positive bacteria retain the violet stain whereas gram-negative bacteria do not. Also called Gram's method.

[After Hans Christian Joachim Gram (1853-1938), Danish physician.]
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.Gram's stain - a staining technique used to classify bacteria; bacteria are stained with gentian violet and then treated with Gram's solution; after being decolorized with alcohol and treated with safranine and washed in water, those that retain the gentian violet are Gram-positive and those that do not retain it are Gram-negative
staining - (histology) the use of a dye to color specimens for microscopic study
References in periodicals archive ?
Failure of Gram's stain to detect Propionibacterium acnes in specimens from clinically significant infections.
Gram's stain results should be consistent with the clinical diagnosis (markedly decreased lactobacillus, predominance of gardnerella, few or no white blood cells).
A Gram's stain of the purulent material in the cyst revealed numerous gram-sensitive cocci and a few gram-negative rods.
If a patient's occupational history and a history of the evolving skin rash make you suspect cutaneous anthrax, do a routine Gram's stain and culture or take a biopsy and culture to make the diagnosis, experts said in an interview.
The application of a tissue Gram's stain or a Warthin-Starry silver stain will detect the gram-negative rods in the foamy macrophages, which are usually numerous and easily identified.
A Gram's stain revealed gram-positive cocci and rare gram-negative rods.
We still recommend a fecal Gram's stain and fecal direct microscopic exam for most patients.
The subset of women who had intermediate-grade bacterial vaginosis at baseline according to Gram's stain scores were more likely than those with high-grade bacterial vaginosis to have lower cure rates if they took lactobacillus and did not become colonized.
The aspirate was evaluated by Gram's stain, aerobic and anaerobic cultures, and Warthin-Starry silver staining (figure 3).
As DNA tests become more accurate, fewer healthcare professionals are relying on the Gram's stain, which is reliable in only about half of all women, and culture testing, which can delay diagnosis by several days.
Surgical tissue Gram's stain and culture were nonrevealing, but fungal culture grew P.