One of the highly researched and used polyguanidines is PHMG made by condensation of hexamethylene diamine with guanidine hydrochloride
However, literature survey has shown that no polymer resin has been synthesized using the monomers thymol, guanidine hydrochloride
, and formaldehyde.
In addition, nanoparticles produced in protein cage can be used as a biosensor, which definitely has high stability because ferritin is able to stand a temperature of up to 80EeAC and a pH value of 2-10 and a 6 M concentration of urea and guanidine hydrochloride
The native state of a protein can be altered using elevated temperature, chaotropic or other chemical agents such as urea or guanidine hydrochloride
, and adjustments in pH.
The reducing agent and buffer components were separated by dialysis against 6 mol/L guanidine hydrochloride
(pH=7) at 4[degrees]C (4), (19).
Protein-disrupting chaotropic agents (urea, guanidine hydrochloride
, and dicyandiamide), surfactants (sodium dodecyl sulfate or cetyltrimethylammonium bromide), and the cosolvent propylene glycol were all expected to provide increased protein-protein and protein-PAE interactions.
They did not generate hazardous wastes containing phenol and chloroform, dispensing the need of a fume hood to be applied, but the use of guanidine hydrochloride
solutions also produces noxious wastes and some care on the disposal of residues should be taken as well.
Scrapie isolates had GdnHCl1/2 values (the concentration of guanidine hydrochloride
able to dissolve half the insoluble [PrP.
Boric acid, guanidine hydrochloride
, sodium metaperiodate, and acetonitrile were purchased from Wako Pure Chemicals Co.
The highly active particulate matter obtained from the ovary was partially solubilized and extracted with a buffer containing 2 M guanidine hydrochloride
, then purified.