The intestinal samples were gradually dehydrated, sectioned at 5 [micro]m and stained with haematoxylin
The sections were stained with haematoxylin
and eosin for microscopic observation of pathological changes in the prostates, using a Nikon 55i microscope (Nikon, Tokyo, Japan).
Sections of 5 pm thick were cut by microtome, stained with haematoxylin
and Eosin and monted on diesterase phthalate xylene.
The specimens were washed in 70% ethyl alcohol, dehydrated, cleared, sectioned and stained with Harris haematoxylin
and eosin stains.
4 to 6m thickness sections were made and stained with Haematoxylin
and Eosin .
and eosin stained sections were analysed by K Pillay and a placental histopathology report was issued.
Whole-mounts were stained using Van Cleave's haematoxylin
with additional drops of Ehrlich's haematoxylin
The best 2 slides from each tissue sample (4 slides per gonad) were stained with a haematoxylin
and eosin stain; coverslips were then mounted with a resin mounting medium.
Gastric tissue samples were fixed in 10% formalin while slides were stained with haematoxylin
and eosin for histopathological examination.
Tongues were cross-sectioned at 4-8 [micro]m (Shandon AS-323, Manchester, UK) and stained with haematoxylin
and eosin (H&E).
Starting at a random distance into the section (between 0 - 500[micro]m), 5[micro]m sections were taken at regular 500[micro]m intervals throughout the lung and stained with haematoxylin
Subsequently, 5-[micro]m thick sections from the mid-sagittal plane of the PPT complex were stained with Safranin O to examine the BTJ proteoglycan profile and with haematoxylin
& eosin (H&E) to examine the general morphology; the latter analysis included an assessment of the tendon collagen fibres under a polarised microscope (Nikon Eclipse 50i, Nikon Inc.