Mapping chromatin states by sequencing immunoprecipitated
chromatin (chip-seq) provides an extraordinary resource for studying cellular states.
Adaptor-ligated DNA was immunoprecipitated
by a monoclonal anti-methylcytidine antibody (Diagenode, USA).
The cells were treated with MG-132 for 6 hours, lysed under denaturing conditions, and immunoprecipitated
with anti-FLAG antibody.
The NRF2 protein was immunoprecipitated
and bound DNA fragments were examined to detect the presence of the putative SPP1 ARE sequence.
B-D) Western blots of immunoprecipitated
serum samples using the Roche M7 antibody (B), the Medimabs N-terminal antibody (C), and the Medimabs C-terminal antibody (D).
After sonication, 5 [micro]g of antibodies recognizing the following AML1 (Santa Cruz Biotechnology, Santa Cruz, USA, sc-28679), ETO (Santa Cruz Biotechnology, Santa Cruz, USA, sc-9737), histone deacetylase 1 (HDAC1) (Abcam, Cambridge, USA, ab7028), DNA methyltransferase 1 (DNMT1) (Abcam, Cambridge, USA, ab13537), DNMT3a (Abcam, Cambridge, USA, ab13888) and DNMT3b (Abcam, Cambridge, USA, ab13604) were immunoprecipitated
with the chromatin overnight.
TABLE 1: Identification of proteins immunoprecipitated
with RPeL27 or RPeL43 in NPC by ESI/QUAD/TOF MS.
The sheared genomic DNA was immunoprecipitated
with normal rabbit IgG (#2729), anti-acetyl-histone-H3 (#06-599) or anti-acetyl-histone-H4 rabbit polyclonal antibody (#06-598) for overnight followed by incubation with Dynabeads protein G (Invitrogen) for 2 h.
phosphotyrosine containing proteins were probed for cortactin levels.
The PGC-1[alpha] protein was immunoprecipitated
in samples containing 200 pg of total protein by using 2 [micro]g of an antiPGC-1 a antibody and 20 [micro]g of protein A plus G agarose beads to determine whether the SIRT1 and PPAR[alpha] proteins coprecipitated with the PGC-1[alpha] protein.
GST pull-down or immunoprecipitated
samples with 50 [micro]g proteins of appropriate lysates were electrophoresed on 8 and 12% SDS-PAGE gels for detection of BRCA1 and RAD51, TP53 proteins, respectively.
The tryptic cleavage product of the mature form of the bovine desmoglein l ectodomain is one of the antigen moieties immunoprecipitated
by all sera from symptomatic patients affected by a new variant of endemic pemphigus.