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Indirubin inhibits LPS-induced inflammation via TLR4 abrogation mediated by the NF-kB and MAPK signaling pathways.
All active ingredients [Baicalin, Liquiritin, Indigotin, Indirubin, Sitosterol, Chlorogenic acid, Matrine, Daidzein, Betaine, Oleanolic acid, Vitamin C, Vitamin E, Folate] were suspended in DMSO and diluted to final concentration 25 umol L-1 in DMEM without FBS and antibiotics as the ligand supplemented media.
Indirubin derivatives inhibit Stat3 signaling and induce apoptosis in human cancer cells.
Beta-sitosterol (MOL000358) exhibits the largest number of target connections (18), followed by Stigmasterol (MOL000449) with 15 potential targets and Acacetin (MOL001689), Tryptantherin (MOL001808) and Indirubin (MOL002309) with 13 potential targets.
The pathogenesis begins with the metabolism of tryptophan to indole, which is absorbed and detoxified to indoxyl sulfate (liver), and finally excreted by bacteria with sulfatase and/or phosphatase that metabolize indoxyl to indirubin (red) and indigo (blue) (3).
1] This condition is characterized by purple discoloration of urine in the urine bag because of the presence of the pigments, indigo and indirubin.
Characterization of a forest soil metagenome clone that confers indirubin and indigo production on Escherichia coli.
For example the bis-indole alkaloid indirubin occasionally present in human urine was amongst the initial cyclin-dependent kinase inhibitor 7].
Indoxyl sulfate is further reduced to indigo or indirubin via an oxidation pathway mitigated by bacterial enzymes.
Indoxyl sulfate was excreted into the urine and metabolized by bacteria to produce indigo (blue) and indirubin (red, [Figure 2]).
The indoxyl turns into indigo which is blue in color and indirubin which is red in color.
Indirubin is a CDKI that has been used in traditional medicine for hundreds of years and is currently being used in clinical trials for CML (19).
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