Lonza Walkersville announced its new offering of Silensomes Human Liver Microsomes
(HLM) products pooled human liver microsomes
for in vitro cytochrome P450 (CYP) phenotyping.
Studies are most frequently performed with (cryopreserved) primary cells or tissue fractions, such as microsomes
, S9 or cytosol, derived from animal organs or human donors (Soars et al.
Lung and OM microsomes
from homozygous 2A13/[2F1-TG.
13) showed that ritonavir is metabolized by CYP 3A4 and CYP 3A5 at the same ratio, but indinavir is metabolized by CYP 3A4 than CYP 3A5 in human intestinal microsomes
and expressed CYP 3A4/3A5.
1,2,4,5] AIH is divided into two types: Type 1 AIH is characterized by circulating antibodies to nuclei (ANA) or smooth muscle (ASMA) and Type 2 AIH is characterized by the presence of antibodies to liver or kidney microsomes
(ALKM-1) and/or to a liver cytosol antigen (ALC-1).
On stipulated day the animals were slaughtered, their livers taken out and microsomes
prepared for the estimation of activities of various membrane bound DME such as cytochrome P450 (CP), cytochrome b5 (CB), cytochrome-c-reductase (CCR), aniline hydroxylase (AH), acetanilide hydroxylase (AAH), benzphetamine demethylase (BD), aminopyrine demethylase (APD), N.
A study describing testosterone metabolism in vitro using fresh and frozen pigskin as well as human skin microsomes
was published in 2014.
were prepared using the method described by Guengerich (1982).
Homogenization of liver samples and isolation of local microsomes
were done as described by Nordlie and Arion .
Examples of protective and antioxidant properties of 1,4-dihydropyridine derivatives have been provided in a number of recent in vitro studies on low density lipoproteins, mitochondria, microsomes
, isolated cells, and cell cultures.
Several studies found an inhibitory effect of [alpha]-naphthoflavone, ketoconazole, troglitazone, tranylcypromine, ebastine, and terfenadine on the rate of astemizole O-demethylation in human small intestinal microsomes
and recombinant CYP2J2 isoform (Matsumoto et al.
In an in vitro study of Relizen's effect on the isoenzyme CYP2D6 in pooled human liver microsomes