osmium tetroxide

(redirected from Osmium tetraoxide)
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osmium tetroxide

n.
A volatile poisonous pale yellow solid, OsO4, with a pungent smell, used as an oxidizing agent and in solution to stain and fix biological material, especially lipids. Also called osmic acid.

osmium tetroxide

n
(Elements & Compounds) a yellowish poisonous water-soluble crystalline substance with a penetrating odour, used as a reagent and catalyst in organic synthesis. Formula: OsO4
References in periodicals archive ?
The washed tissues were then allowed to undergo post-fixation with 1% Osmium tetraoxide for 2 hours at 4[degrees]C, dehydrated with graded ethanol (30%, 50%, 70%, 90% and 100%) and dried with liquid C[O.
Samples were post-fixed in 2% aqueous solution of osmium tetraoxide for 4h, then dehydrated in a series of graded alcohols and finally dried to a critical drying point with a Critical Point Dryer unit.
Tissues were transferred to a 1% solution of osmium tetraoxide prepared with Millonig's, fixed for 2 hours, and then washed two times with Millonig's.
5% solution of glutaric aldehyde on the phosphate and cacodylate buffer, finished fixing with 1% solution of the osmium tetraoxide after dehydration in the alcohol-acetone, then dried with method of critical point in the apparatus HCP-2 and were pulverized with the gold in the apparatus IB-2.
After fixation, the cells were covered with 1% osmium tetraoxide (OsO4) in 0.
The examined samples were first cut under cryogenic conditions with a Leica Ultracut microtome with a diamond knife to a thickness of about 70 nm and tinted with osmium tetraoxide.
Washing in several changes of buffer for 30 minutes was then followed by post fixation in 1 percent osmium tetraoxide in cacodylate buffer for 4 hours at room temperature.
4 at 4[degrees]C during 3 hours; they were washed with the same solution during 10 minutes and were then post-fixed in osmium tetraoxide at 1% in buffer cacodylate during 1 hour.
5 % gluteraldhyde and 200 ul of a 1% osmium tetraoxide each of which was suspended in the sodium/magnesium buffer at pH 7.