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polymerase chain reaction
(redirected from PCR reaction)

   Also found in: Medical, Encyclopedia, Wikipedia 0.01 sec.
polymerase chain reaction
n. Abbr. PCR
A technique for amplifying DNA sequences in vitro by separating the DNA into two strands and incubating it with oligonucleotide primers and DNA polymerase. It can amplify a specific sequence of DNA by as many as one billion times and is important in biotechnology, forensics, medicine, and genetic research.


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For standard PCR reactions, they are available in natural tubes with domed caps, while for fluorescent applications, such as QPCR (quantitative PCR), Ultra Clear Caps (available in both white and natural tubes) provide an optically clear window into the reaction for enhanced detection of the fluorescent signal.
A 4-[micro]L aliquot of each PCR reaction was checked for the presence of a specific amplification product by agarose gel electrophoresis (2% agarose, tris acetate EDTA [TAE gel], 50 volts) and ethidium bromide staining.
The target for the Bordetella pertussis PCR reaction is also found in Bordetella holmesii.
 
 
 
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