DPPH is a stable free radical with absorption at 570nm, exhibits a deep purple color in methanol solution which gets reduced to a yellow colored product diphenyl picryl
Crude metabolites of Aspergillus terreus (JAS-2) was evaluated for antioxidant assay by using DPPH (2, 2-diphyenyl 2 picryl
hydrazyl) free radical scavenging method .
The antioxidant compounds react with the radical and convert to picryl
hydrazine from as evidenced through discoloration (from purple to yellow).
1 Qualitative DPPH radical scavenging assay using thing layer chromatography Qualitative screening for antioxidant activity was done using the 1,1-diphenyl-2 picryl
hydrazyl (DPPH) (Sigma-Aldrich) radical assay.
The use of the stable free raadical diphenyl picryl
hydraz (DPPH) for estimating antioxidative activity'.
chloride-induced delayed type hypersensitivity (PC-DTH) response in mice was depressed after the implantation of tumor and treatment with 5-FU.
The evidence of the presence of these radicals due to ultrasonic exposure was obtained by carrying out the sonication in the presence of unsaturated, polymerizable monomers by the trapping of the radicals using radical scavengers such as DPPH ([alpha],[alpha]'diphenyl picryl
We obtained cyclosporin A from Sandoz, HPLC-grade methanol from Jiangsu Hanbon Science and Technology, S-adenosyl-L-methionine and 2,4,6-trinitrobenzene-sulfonic acid hydrate (TNBS) from Sigma-Aldrich, Sephadex LH-20 from Amersham Pharmacia Biotech, silica gel from Merck, and picryl
chloride from Nacalai Tesque.
Oxygen, sulfur, cucl2, Fecl3, p-benzoquinone, nitrobenzene, DPPH(diphenyl picryl
hydrazyl) could scavange the radical used in the inks and coatings.