According to Suad, the most challenging piece to make was Porpita porpita
, which was inspired by a picture of a blue marine organism that she saved for future inspiration.
Specimens of Chrysaora quinquecirrha (Desor, 1848) (Class Scyphozoa), Physalia physalis (Linnaeus, 1758), Porpita porpita (Linnaeus, 1758) Cladonema (sp.
In Chrysaora, Cladonema, Physalia, and Porpita, the staining was particularly dense with the anti-FMRFa antibody.
In the two siphonophore species examined, Porpita and Physalia, the tentacles bear large assemblies of cnidocytes, called cnidosacs, that appear as a line of hemispheres or spheres arranged along one edge of each tentacle.
The cnidosacs in Porpita were small enough to be fully reconstructed from confocal optical slices and provided a measure of the overall density of sensory neurons.
In Physalia, the cnidosacs are more hemispherical and the ectoderm thinner than Porpita.
Irrespective of the organization of the cnidocytes, be they in small planar clumps (Chrysaora), broad circumtentacular bands (Chiropsalmus), single capitate bulbs (Cladonema), or the linear arrangement of nearly cnidosacs found in Physalia and Porpita, a dense plexus of FMRFamide IR neurons is present around the base of the cnidocytes.