Mouse 3T3-L1 preadipocytes
were cultured in DMEM supplemented with 10% FCS and 100 U/ml of penicillin, 100 [micro]g/ml of streptomycin, 250 ng/ml Amphotericin B.
The primary objective of this study was to demonstrate the effects of saturated fatty acids (palmitic and stearic acid) and unsaturated fatty acids (oleic and linoleic acid) on specific gene expression in bovine satellite cells (BSC) and intramuscular preadipocytes
Resveratrol induces Sirt1-dependent apoptosis in 3T3-L1 preadipocytes
by activating AMPK and suppressing AKT activity and survivin expression.
We believe that the multicellular interaction and growth arrest of preadipocytes
and the adipose-derived stem cells will promote differentiation, thereby enhancing the expression of adiposespecific functionality.
1 Subcutaneous Preadipocytes
and Adipocytes 255 14.
D-Delta-tocotrienol suppresses the differentiation of murine 3T3-F442A preadipocytes
The fungicide triflumizole also acts through PPAR[gamma] to induce adipogenesis in MSCs and preadipocytes
in vitro through a PPAR[gamma]-dependent mechanism and promotes increased WAT depot size and altered MSC programming in vivo (Li et al.
Considering that oxidative stress leads to a release of zinc from MT and to cellular zinc depletion, this condition as well as zinc deficiency due to decreased absorption, increased excretion or increased requirements could possibly lead to diabetes mellitus Furthermore, zinc increased phosphorylation of serine residues and therefore activation of Akt in preadipocytes
and adipocytes thereby enhancing GLUT translocation.
Adipose tissue engineering strategies have commonly involved the use of seeding preadipocytes
on appropriate polymeric scaffolds.
Although adipocytes are the main cells in WAT, this tissue also contains preadipocytes
(adipocytes precursor cells, 15-50%), macrophages, fibroblasts, leukocytes and many collagen fibers, which provide structural support.
Macrophage-secreted factors promote a profibrotic phenotype in human preadipocytes
Over a period of 10 days or more, immature fat cells, called preadipocytes
, go through several stages to become mature fat cells, or adipocytes.