restriction enzyme

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restriction enzyme

n.
Any of a group of enzymes that catalyze the cleavage of DNA at specific sites to produce discrete fragments, used especially in genetic engineering. Also called restriction endonuclease.

restriction enzyme

n
(Biochemistry) any of several enzymes produced by bacteria as a defence against viral infection and commonly used to cut DNA for genetic manipulation or diagnosis

restric′tion en`zyme


n.
any of a group of enzymes that are capable of cutting DNA at specific sites along its strand.
[1960–65]
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.restriction enzyme - any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology
endonuclease - a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
References in periodicals archive ?
Golden Gate is a method that utilizes Type IIS restriction enzymes to allow scientists to assemble up to nine DNA fragments in a single step.
2007) used various restriction enzymes like AluI, BsaJI, and Rsal to identify pork from different mixtures of meat; chicken, pig and cattle in various modifications.
The restriction endonucleases AseI, MaeI, MboII, TaqI, and BsmAI were selected for the first PCR product (for the 709-bp amplified product using primers LCO1490 and HC02198), and the restriction enzymes BsaJI, NlaIV, MaeII, TfiI, and BfaI were selected for the second product (for the 597-bp amplified product using primers rntd-10 and C/N-2769) based on the best discrimination and species-specific amplicon patterns.
A person must interfere in the process to obtain the required sequences of actions through many restriction enzymes.
132) and learning to cut and paste genes using chemical scissors called restriction enzymes (3/20/76, p.
These molecules - known as restriction enzymes - control the speed at which bacteria can acquire resistance to drugs and eventually become superbugs.
Each genomic DNA was having one or few sites for restriction enzymes.
Restriction digestion analysis: The 1080 bp and 768 bp PCR products of TEM and SHV genes respectively were digested with restriction enzymes BSeG1, Pst 1and EcoR 1 (Amersham Pharmacia Biotech, USA) according to manufacturer's recommendations.
What makes it stand out is the fact that this is the first solution in the market that not only supports all kinds of restriction enzymes (Type I, II and III) but also takes into account real time laboratory conditions (i.
Because this lesson utilizes RFLP analysis, a thorough review of nucleotide pairing rules will provide the necessary framework for introducing the concept of restriction enzymes later in the presentation.

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