reverse transcriptase

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reverse transcriptase

n.
A polymerase that catalyzes the formation of DNA on an RNA template, found in the retroviruses and in certain other viruses, such as hepatitis B virus.

reverse transcriptase

(trænˈskrɪpteɪz)
n
(Biochemistry) an enzyme present in retroviruses that copies RNA into DNA, thus reversing the usual flow of genetic information in which DNA is copied into RNA

reverse′ tran•scrip′tase

(trænˈskrɪp teɪs, -teɪz)
n.
a retrovirus enzyme that synthesizes DNA from viral RNA, the reverse of the usual DNA-to-RNA replication: used in genetic engineering.
[1970–75]
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.reverse transcriptase - a polymerase that catalyzes the formation of DNA using RNA as a template; found especially in retroviruses
polymerase - an enzyme that catalyzes the formation of new DNA and RNA from an existing strand of DNA or RNA
References in periodicals archive ?
The RNA was subsequently reverse transcribed into complementary DNA (cDNA).
Total RNA from each sample was reverse transcribed into cDNA with an oligo (dT) primer.
RNA was reverse transcribed by using the Superscript III Platinum OneStep Real Time RTPCR system (Invitrogen, Carlsbad, CA, USA) and subjected to PCRs specific for DENV, CHIKV, and ZIKV using the CDC Trioplex Real-time RT-PCR Assay.
RNA was extracted and reverse transcribed (2 mcg) and reverse transcription polymerase chain reaction analysis was carried out for SOD expression as described in materials and methods.
RNA was reverse transcribed by using the SuperScript II Reverse Transcription Kit (Invitrogen, Carlsbad, CA, USA) and subjected to PCRs specific for DENV (5) CHIKV (6), WNV (7) and Mayaro virus (8).
Because of difficulties with reverse transcribing the entire SINV-3 genome (wild type or recombinant), 3 regions of the SINV-3 genome were reverse transcribed and amplified to verify transcript production.
The extracted RNA was reverse transcribed to make cDNA through reverse transcriptase enzyme (M-MLV) using PCR.
The pathologist reverse transcribed HCV-RNA to cDNA using 100 U of M-MLV reverse transcriptase at 37C for 50 minutes.
After 48 h stimulation and 24 h recovery, mRNA was extracted and reverse transcribed.
A band corresponding to ~1117 bp is seen (Figure 3, panel A, lane 3) when RNA extracted from null cells of Dictyostelium was reverse transcribed and subjected to PCR amplification.

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