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Southern blot analysis
(redirected from Southern blot)

   Also found in: Medical, Encyclopedia, Wikipedia 0.01 sec.
Southern blot analysis
n.
A technique for identifying specific sequences of DNA in which DNA fragments are separated by electrophoresis, transferred to nitrocellulose, and identified with a suitable probe.

[After Edwin Mallor Southern (born 1938), British biochemist.]


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The diagnosis of B-cell lymphoid malignancy can frequently be substantiated by detecting clonal immunoglobulin heavy chain (IGH) gene rearrangements, which is typically done by polymerase chain reaction (PCR) amplification and/or Southern blot analysis.
Plasmid DNA was not detected in any of these preparations, either when analyzed by agarose gel electrophoresis or after Southern blot hybridization analysis with a [bla.
False negatives in this study were excluded by subsequent analysis with the Southern blot technique.
 
 
 
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