Southern blot

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Related to Southern blot: Northern blot

South′ern blot′

a test for the presence of a specific DNA segment in a sample by observing whether single strands of DNA from the sample will bond with labeled strands from a known segment of the DNA in question.
[after Edwin M. Southern, originator of the test]
References in periodicals archive ?
Integration of the MsHsp23gene into the alfalfa plant genomes was further confirmed with Southern blot analysis.
Southern blot hybridization was performed using DIG High Prime DNA Labeling and Detection Kit 2 (Roche Molecular Systems, Pleasanton, CA, USA).
RNase A (10[micro]g/well) was added to the lysed cells and genomic DNA was isolated from each cell line using the PI-1200 DNA isolator (Kurabo, Osaka, Japan) for Southern blot analysis.
To corroborate the position of inserts in the restriction fragments, Southern blot hybridization was fulfilled using pMC505 as probe (data not shown).
The RFLPs could then be used within affected families to trace the X chromosome carrying the mutant gene using the Southern blot technology.
Common mtDNA mutation was detected by multiplex PCR and telomere shortening was tested by Southern blot analysis.
The study further used southern blot analysis and immunofluorescence to analyse the alternative lengthening of telomeres mechanism.
As established by Pfister et al (10) and Ozden et al, (4) HPV subtypes 13 and 32 have been identified as the cause for FEH lesions through polymerase chain reaction (PCR) analysis and Southern blot hybridization, respectively.
S1 nuclease pulsed-field gel electrophoresis and Southern blot analysis were used to identify the sizes of [bla.
The presence of begomoviruses was also confirmed by Southern blot analysis using cloned DNA-A probe of Cotton leaf curl virus.
Con el fin de detectar el elemento dTdic1 en el DNA genomico de clavel, se realizo Southern blot utilizando el Kit Dig High Prime Labeling and Detection Starter Kit II de ROCHE[R], siguiendo el protocolo descrito en DIG Application Manual for Filter Hibridization de ROCHE[R].

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