Southern blot

(redirected from Southern blot analyses)
Also found in: Medical.
Related to Southern blot analyses: Northern blot analysis, RFLP, Western blot analysis

South′ern blot′



n.
a test for the presence of a specific DNA segment in a sample by observing whether single strands of DNA from the sample will bond with labeled strands from a known segment of the DNA in question.
[after Edwin M. Southern, originator of the test]
References in periodicals archive ?
Southern blot analyses of the plants inoculated with TYLCV-OM Rad-1 and ChiLCV- OM Rad-2 showed a high accumulation of both viruses (Fig 4, panels AI and AII).
High-resolution methylation polymerase chain reaction for fragile X analysis: evidence for novel FMR1 methylation patterns undetected in Southern blot analyses.
The published work, titled "High-resolution methylation polymerase chain reaction for fragile X analysis: Evidence for novel FMR1 methylation patterns undetected in Southern blot analyses," describes the accurate and reproducible assessment of the methylation status in 80 clinical samples, including 39 full mutation specimens, with concordance to the reference method of Southern blot analysis.
Purified PCR amplification product of aiiA gene served as a probe to be used in the Southern blot analyses.
Ten micrograms of total genomic DNA were digested in parallel with positive control samples (plasmid PV-ZMIR13 DNA spiked into 10 [micro]g of nontransgenic corn DNA) and subjected to Southern blot analyses.

Full browser ?