polymerase

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pol·y·mer·ase

 (pə-lĭm′ə-rās′, -rāz′, pŏl′ə-mə-)
n.
Any of various enzymes, such as DNA polymerase, RNA polymerase, or reverse transcriptase, that catalyze the formation of polynucleotides of DNA or RNA using an existing strand of DNA or RNA as a template.

polymerase

(pəˈlɪməreɪz)
n
(Biochemistry) any enzyme that catalyses the synthesis of a polymer, esp the synthesis of DNA or RNA

pol•y•mer•ase

(ˈpɒl ə məˌreɪs, -ˌreɪz)

n.
any of several enzymes that catalyze the formation of a long-chain molecule by linking smaller molecular units.
[1955–60]
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.polymerase - an enzyme that catalyzes the formation of new DNA and RNA from an existing strand of DNA or RNA
enzyme - any of several complex proteins that are produced by cells and act as catalysts in specific biochemical reactions
DNA polymerase - the enzyme responsible for DNA replication
RNA polymerase, transcriptase - the enzyme that copies DNA into RNA
reverse transcriptase - a polymerase that catalyzes the formation of DNA using RNA as a template; found especially in retroviruses
References in periodicals archive ?
1 mM each of the deoxynucleotide triphosphates; 30 mg/L bovine serum albumin, 50 mL/L dimethyl sulfoxide, 2 [micro]L of 10x Taq Buffer, 1 [micro]L of the sample, 1 U of Taq polymerase, and assay-specific amounts of Mg[Cl.
A standard PCR reaction mixture (20 ng of human cDNA/50 [micro]L) for multiplex amplification reactions was formulated for amplification of two targets on the iCycler iQ, using FastStart Taq polymerase (1.
1 mM of each primer (Eurofins, Canada), PCR buffer (Fermentas, USA), 200 mM of each dNTP, 2 U of Taq polymerase (Fermentas, USA) and 200-500 ng of DNA template.
The method uses a thermophilic RNase H2 enzyme from Pyrococcus abyssi, which functions optimally in conditions compatible with thermophilic DNA polymerases, such as Taq polymerase.
Amplification of DNA is done with the help of specific primers (GP5+/GP6+), dNTPs, Taq Polymerase for the detection of HPV DNA.
Auto claved distilled water X ul and Taq polymerase 1 unit was added.
5 units Taq polymerase (Thermo Scientific) and 1uL of extracted DNA.
In RT-2 reaction, denatured dsRNA was then added to the reaction mixture consisting of 10 l of the 5X reaction buffer, 1 l of 10 mM deoxynucleoside triphosphate (10mM dNTP) mixture, 2 l of 25 mM MgSO4, 1 l of MuLV Reverse Transcriptase enzyme (5U/ul), 1 l of Taq Polymerase (5U/ul) and 3 l of dimethyl sulfoxide in a final volume of 50 l and then preincubated at 480C for 45 min followed by 35 cycles of 940C for 1 min.
As it can be seen from the results of electrophoresis, Taq polymerase is capable to effectively incorporate in the sequence of a strand only modified nucleotide 5-(C8 Alkyne)-2'-Deoxyuridine-5'-Triphosphate.
Amplification of the mt cyt b gene was performed in a final volume of 25 [micro]L containing 250 ng of extracted DNA, mega-mix royal (optimized mixture of Taq polymerase, anti-Taq polymerase monoclonal antibodies in 2*reaction buffer (6 mM Mg[Cl.
5 U of Taq polymerase (Fermantas, USA), 5 L PCR buffer (50 mM KCl, 10 mM Tris-HCl, 1.