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2% tetrazole solution, in dark chamber at 35[degrees]C for 2 h, after which the seeds were washed and the reading was immediately performed, classifying the seeds as viable (dormant) and non-viable (dead) (Novembre et al.
x 3H2O, RPMI, 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide, yellow tetrazole (MTT), penicillin-streptomycin, DAPI (4_,6-diamidino-2-phenylindole, dihydrochloride), ethidium bromide and Acridine orange were all purchased from Sigma-Aldrich.
VST has two proton dissociating groups, a carboxyl group and a tetrazole, in the structure, and these parts contribute to the aqueous solubility [12, 13].
Lee, Synthesis of Polymers Including Both Triazole and Tetrazole by Click Reaction, Bull.
Interactions of pentamethylenetetrazole and tetrazole analogues with the picrotoxinin site of the benzodiazepine- GABA receptorionophore complex.
The team further modified this polymer by substituting tetrazole groups on to the monomer.
Tetrazole derivatives possess broad spectrum of biological activity in both medicinal and pharmaceutical such as antibacterial, antifungal, antiviral, analgesic anti-inflammatory, antiulcer [6-13].
At the time, the tetrazole chemotype (rightmost above) was pursued and patents based on this scaffold were filed.
Chemicals include; p-toluene sulphonyl semicarbazide, zinc carbonate and 5-phenyl tetrazole.
Some specific topics covered in later chapters are the photochemistry and applications of benzoindoles and piridoindoles, 2+2 cycloaddition on furan derivatives, photochemistry in ionic liquids, and photochemistry of tetrazole derivatives in cryogenic rare gas matrices.