LAST time in my A-Z of plants we looked at Ulex europaeus
The epithelial component is positive for low- and high-molecular-weight cytokeratins and Ulex europaeus
Then slides incubated with Ulex europaeus
agglutinin (UEA-I) and peanut agglutinin (PNA) peroxidase conjugated lectins at room temperature for 2 hr, were washed in PBS solution for 20-30 min, then placed in DAB-[H.
The immunohistochemical expression (yes/no) of several markers was evaluated; these were vimentin, cytokeratin (CK), CD117, CD68, CD45, CD34, CD31, CD10, epithelial membrane antigen (EMA), desmin, smooth muscle actin (SMA), Factor VIII, S100, Ulex europaeus
agglutinin, I (UEA), carcinoembryonic antigen (CEA), actin, melan-A, HMB45 and vascular endothelial growth factor (VEGF).
Then cells were stained with FITC-labellecI lectin from Ulex europaeus
(FITC-UE lectin, Sigma).
Because of the indications of increased [beta]1-6 branching of N-glycans and [alpha]1-2 fucosylation as aberrant glycosylation in prostate cancer progression, we used the lectins phytohemagglutinin-L (PHA-L) and Ulex europaeus
agglutinin (UEA), which preferentially recognize the [beta]1-6 branched N-glycans and the [beta]1-2 fucosylated N-glycans, respectively, in the multiplex lectin immunosorbent assays (LISAs).
Multiple investigators have examined vascular invasion in MPUC by using immunohistochemistry for factor VIII, Ulex europaeus
, CD31, and CD34, (1,7-10) as summarized in Table 1.
Most IHC studies have shown that KS lesional cells express endothelial antigens based upon their immunoreactivity for Ulex europaeus
agglutinin-1 (UEA-1), thrombomodulin, factor VIII-related antigen (von Willebrand factor), CD31 (also called PECAM-1 for platelet endothelial cell adhesion molecule) (Figures 6A,B), and CD34 [81,82].
The general vegetation around the nesting site was dense, low (<5 m) native scrub ("Matorral Nativo") (Asociacion Bogotana de Ornitologia 2000) with a few dispersed plants of Cytisus monspessulanus, Ulex europaeus
, and Oxalis minutiflora.
This differentiation is generally detected by seed extracts of Ulex europaeus
5 or alpha smooth muscle actin in conjunction with Ulex europaeus
agglutinin 1 lectin to fully label the pulp vasculature.