The second objective aims at clarifying the basis of prion neurotoxicity and will be developed along two alleys: (a) we will uncover the molecular basis of spongiosis (the neuronal vacuolation
characteristic of prion diseases), which we suspect to be a main driver of pathology, and (b) we will perform CRISPR-based synthetic lethality screens to identify genes that become essential to prion-infected cell lines (which do not experience prion toxicity) and may not be expressed by neurons.
Histopathological analysis of liver sections showed large areas of hepatic parenchymal necrosis, extensive vacuolation
congestion and bile duct proliferation in the animals treated with triptolide alone (Fig.
Moreover, they exhibited irregularly-shaped nuclei, irregular distribution of cytoplasmic organelles and vacuolation
(Figures 5D, 5F and Figure 6B).
Changes observed in animals following dichloromethane exposure include liver foci/ areas of alteration, hepatocyte vacuolation
of lipids in the hepatocyte), fatty liver, and necrosis, with effects seen beginning at 500 ppm (Burek et al.
A single section from all inoculated mice was stained with hematoxylin and eosin to determine the presence and severity of disease-specific vacuolation
in 9 standard gray matter regions and 3 white matter regions, a protocol referred to as lesion profiling (18).
F, Case 173 (SI00 negative: primary melanoma composed of highly atypical cells with some "rhabdoid" features (arrows), displaying eosinophilic cytoplasm, slight lateral nuclear displacement, and focal nuclear vacuolation
(hematoxylin-eosin, original magnifications X200 [A, B, E, and F] and X400 [C and D]).
Distal motor axonopathy and central nervous system myelin vacuolation
caused by cycloleucine, an inhibitor of methionine adenosyltransferase.
or spongiform degeneration in the grey matter of the six cortical layers of the cerebral cortex or within the molecular layer of the cerebellar cortex is the hallmark pathological feature of CJD.
Brain, spinal cord and retina are considered the target organs in which the virus actively replicates causing extensive tissue vacuolation
Additionally, the neutrophils displayed a left shift with toxic vacuolation
2005), working with Araucaria angustifolia (Parana pine) callus, observed some elongated vacuolated cells, with vacuolation
defined as an early marker of cell death (FILONOVA et al.
Other findings within the central nervous system included multifocal vacuolation
and separation of white matter fibers consistent with spongiosis throughout the spinal cord.