Histopathological and Transmission electron microscope procedures: The overnight fasted rats, were sacrificed in the morning under ether
anaesthetization and dissected to acquire the kidneys which were fixed in 10 % saline buffered formalin for histological examination and were fixed immediately with 2.5 % glutaraldehyde and 2 % paraformaldehyde for transmission electron microscope investigation (Bancroft & Gamble, 2008).
Reduced initial state of stress could thus permit lighter dosages with still adequate
anaesthetization and safe manipulation, which, in turn, will likely produce lighter long-term stress effects as well.
Comparative efficacy of clove oil and other chemicals in
anaesthetization of Pomacentrus amboinensis, a coral reef fish.
Shell height was negatively correlated with
anaesthetization success rate, with small oysters more likely to open their shell in response to MgS[O.sub.4].
As a noncompetitive NMDA receptor antagonist, ketamine has been used for
anaesthetization since the 1960s and was reported in 2000 to rapidly improve depressive symptoms, including refractory depression within several hours.
Briefly, the rats received surgeries under
anaesthetization at day 21 and their eyes were then enucleated.
Experimental surgeries: After
anaesthetization, the animals were restrained in dorsal recumbency.
There was good
anaesthetization with sensory and motor blockade of the saddle area.