FAS - a congenital medical condition in which body deformation occurs or facial development or mental ability is impaired because the mother drinks alcohol during pregnancy
We wanted to further elucidate the kinetics of activation markers and cosignaling receptors on the cultured cells and thus analyze the costimulation marker C[DELTA]28, the activation marker CD69, the activation/proapoptosis marker CD95, and the coinhibitory receptors CTLA-4, PD-1, LAG-3, and TIM-3.
At the same time, the increased production of ROS in RECEs can induce the apoptosis by activating the JNK signaling pathway of the apoptosis system cluster of differentiation 95 (CD95)/CD95 ligand (CD95L).
Fluorochrome- or biotinlabeled human monoclonal antibodies specific for the following antigens were purchased from BD Biosciences (San Jose, CA, USA), eBioscience (San Diego, CA, USA), and BioLegend (San Diego, CA, USA): CD8a (SK1), CD4 (L200), CD3 (SP342), CD28 (CD28.2), CD95 (DX2), CD1b (SN13), CD8[beta] (SIDI8BEE), HLA-DR (G46-6), CXCR5 (MU5UBEE), and PD-1 (EH12.2H7).
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