The most common antibiotic that the staphylococci isolates were resistant to was penicillin 206 (80.42%); followed by fusidic acid 204 (72.4%), erythromycin 153 (54.45%), amoxicillin 78 (27.76%); tetracycline 74 (26.33%); oxacillin 70 (24.91%);
cefoxitin 63 (22.42%); mupirocin 41 (14.59%); gentamycin 27 (9.61%); cefepime 20 (7.12%), and chloramphenicol 14 (4.98%).
Therefore, routine phenotypic validation tests must be performed to distinguish
cefoxitin resistance due to loss of porin from the presence of AmpC enzyme and to prevent masking of AmpC's presence of extended-spectrum beta-lactamase (ESBL).
CoNS isolates were submitted to a disc diffusion susceptibility test using the following antibiotics: penicillin (10 [micro]g), oxacillin (1 [micro]g),
cefoxitin (30 [micro]g), erythromycin (15 [micro]g), clindamycin (2 [micro]g), rifampicin (15 [micro]g), gentamicin (15 [micro]g), ciprofloxacin (5 [micro]g), tetracycline (30 [micro]g), vancomycin (30 [micro]g), linezolid (30 [micro]g), ceftazidime (30 [micro]g), sulfamethoxazole-trimethoprim (23.75/1.25 [micro]g) and novobiocin (Oxoid).
Keywords: Methicillin resistant Staphylococcus aureus, oxacillin,
cefoxitin, PCR.
aureus isolates to penicillin,
cefoxitin, erythromycin, clindamycin, quinupristin-dalfopristin, linezolid, trimethoprim-sulfamethoxazole, amoxicillin-clavulanate, ciprofloxacin, ceftriaxone, tetracycline, gentamicin, and rifampin was determined by disk diffusion test according to the Clinical Laboratory Standards Institute (CLSI) guidelines.
The patient was admitted for intractable pain and fever and started on intravenous
cefoxitin 2 g IV every 8 hours and oral doxycycline 100 mg every 12 hours for pelvic inflammatory disease vs tick- or flea-borne illness.
While oxacillin and
Cefoxitin were 100% (21) resistant for MRSA and sensitive for MSSA.
Material and Methods: Modified Three Dimensional Test (M3DT) taken as Gold standard, Modified Hodge Test (MHT) (
Cefoxitin), Nitrocephin test and three screening strategies for the detection of Amp C Beta-lactamases were tested on urinary isolates of E.
An identification-confirmed Klebsiella pneumoniae with reduced susceptibility to
cefoxitin may suggest porin mutation or acquisition of plasmid-coded AmpC [beta]-Lactamase.
[9] Antibiotic discs used were amoxycillin/ clavulanic acid (20/10 [micro]g), piperacillin-tazobactam (100/10 [micro]g), gentamicin (10 [micro]g), amikacin (30 [micro]g), ciprofloxacin (5 [micro]g), trimethoprim-sulfamethoxazole (1.25/23.75 [micro]g), ceftazidime (30 [micro]g), ceftriaxone (30 [micro]g), cefotaxime (30 [micro]g),
cefoxitin (30 [micro]g), imipenem (10 [micro]g) and cefuroxime (30 [micro]g).
(12,13) All test isolates were subjected to the antimicrobial activity of
cefoxitin disk (30 [micro]g) on aseptically streaked MH agar plates.