The main reason for these high variations can be attributed to the
cell plate materials, especially the cover, which attenuates the beam.
Then, neutral red solution (1%, 100L/well) was added to the
cell plate. After further incubation for 60 min, the cells were washed three times with RPMI-1640 medium to remove excess neutral red.
SAR distribution at 1.8 GHz across the bottom layer of the biological sample obtained for different size of aperture drilled in the lower TEM
cell plate without ITO layer.
Standard was then added to columns 1 and 2 on the
cell plate followed by 25 [micro]L of cells into the remaining wells.
When ASM cells were cultured into monolayer, the serial twofold dilutions of drugs and MP solution were added to
cell plate, respectively, in four manners.
The fundamental nature of the BMA was described (Brown & Lemmon, 1988b, 1993) in the unique AMS of homwort mitosis where the AMS orients parallel to the long axis of the dividing plastid and spindle and at right angles to the division plane and
cell plate. This basic structure can explain the construction of the far more complex QMS in meiosis.
TEM would be necessary to determine if the structure shown is a concentration of organelles or actually a partial
cell plate resulting from some degree of vesicle fusion.
In the cited work [22], hard anchoring conditions for the nematic on the
cell plates has been assumed; this means that the orientation of the molecules at the plates is not affected by the applied electric field.