Microsatellite data analysis: Popgene 1.32 (Yeh and Boyle 1997) was used to estimate the number of alleles (NA) and effective number of alleles (NE), and CERVUS 3.0 (Kalinowski et al., 2007) was employed to calculate the polymorphic information content (PIC), expected
heterozygosity (HE) and observed
heterozygosity (HO).
These features include the analysis of human leukocyte antigen loss of
heterozygosity, the presence (or absence) of seven of the most common oncogenic viruses, as well as the composition of the T-cell receptor alpha repertoire, which is complementary to the previously-released TCR beta analysis.
Statistical analysis for calculation of genetic variability measures such as number of alleles, expected and observed
heterozygosity, homozygosity and Polymorphic Information Content (PIC) was calculated for all microsatellite markers.
The number of alleles (NA), observed (HO)
heterozygosity and expected (HE)
heterozygosity were calculated using ARLEQUIN ver.
The converted data was processed using POPGEN version 1:32 program [11] to generate the observed number of alleles ([n.sub.a]), effective number of alleles ([n.sub.e]), observed
heterozygosity value ([H.sub.o]), expected
heterozygosity value ([H.sub.e]), genetic differentiation ([F.sub.ST]), the global heterozygote deficit among breeds ([F.sub.IT]), and the heterozygote deficit within the breed ([F.sub.IS]), gene flow (Nm), Hardy-Weinberg equilibrium (HW), and allele frequency.
The number of allele, and expected and observed
heterozygosity were characterized in the wild population of Panjin, China.
The three DNA abnormalities examined in the study, all of which are included in the PancraGEN(R) assay, included: elevated DNA quantity, KRAS mutation, and loss of
heterozygosity mutations among a panel of key tumor suppressor genes.
Arlequin software version 3.5 was used to calculate allele frequencies and to obtain expected
heterozygosity (He), observed
heterozygosity (Ho) and probability value (p-value) of Hardy-Weinberg equilibrium (7).
In this present study, the cultured population showed a higher level of inherent genetic diversity and allele richness than the wild as revealed by indicators such as the percentage of polymorphic loci (%P), Number of Alleles (Na), Number of effective alleles (Ne), Shannon Information index (I), and expected
heterozygosity (Nei's gene diversity).
According to Hardy-Weinberg, and to the fixation index (f), the intrapopulation genetic variability has been quantified through the number of alleles per locus (A), the percentage of polymorphic loci (P) and the observed (Ho) and expected (He)
heterozygosity (Ho) (Hamrick, 1983).
Different measurements of within breed genetic variations like observed and expected
heterozygosity, Shannon index and polymorphic parameters (the number of actual alleles and the number of effective alleles) were calculated using POPGENE software package (Yeh et al., 1999).