o IL-1?, IL-1?, IL-2, IL-4, IL-5, IL-6,
IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-23, IFN-?, TNF-?, TNF-?
After 24 h of treatment, we collected the PBMCs and the supernatants and then determined the protein or mRNA levels of cytokines, including IL-1[sz], IL-33, IL-6,
IL-8, and tumor necrosis factor-a (TNF-a).
Silencing of endogenous IL-32 in differentiated THP-1 human macrophages significantly decreased TNF-[alpha], IL-1[beta], and
IL-8 production and simultaneously increased the M.
To further elucidate the pathogenesis of IRIS, we analyzed gene expression changes of NFAT1-NFAT4 and the major target genes IL-2, IL-6,
IL-8, TNF-[alpha], IL-1[beta], IL-10, and IL-12 in peripheral blood [CD3.sup.+] T cells before and during the course of IRIS.
IL-6, TNF,
IL-8 monoclonal antibodies, standard samples, and negative controls were provided by Professor Jin Boquan from the Department of Immunological Teaching and Research of the Fourth Military University.
A no primary antibody, B no treatment, C, IL-15 (100ng/ml); D,
IL-8 (100ng/ml); E, IL-1b/TNF-a (100ng/ml).
Further, colocalization between
IL-8 and Fc[gamma]RIIa could be blocked by anti-Fc[gamma]RIIa.
A simple method for determining cell-associated
IL-8 was described by Marie et al.
In addition, keratinocytes in psoriasis as well as synoviocytes in RA are capable of responding to direct IL-36 ligands stimulation with production of IL-6,
IL-8, and antimicrobial peptides, which cooperate with IL-17A and TNF-alpha promoting neutrophil activation and migration [11, 54, 56, 60].
The potential roles of cytokines, IL-5 and
IL-8, and plasma cortisol in the anti-inflammatory actions of phosphodiesterase inhibitors in sensitized guinea-pig airways.
Interleukin-8 (
IL-8) is a cytokine produced by a variety of cells in response to stimuli, such as bacterial lipopolysaccharide, with a strong chemotactic activity on neutrophils.