First detection of Leishmania infantum
kinetoplast DNA in hair of wild mammals: application of qPCR method to determine potential parasite reservoirs.
Also we have already assessed the sensitivity of two methods of semi-nested for minicircle
kinetoplast (kDNA) and nested PCR of ITS-rDNA to detect Leishmania parasites in Phlebotomus papatasi which revealed the high infection rates of L.
A multiple genetic marker approach (mini-circle
kinetoplast DNA, restriction analysis of 3'UTR of HSP70 and HSP70 gene sequence analysis) was adopted (10) for the same.
Ziyaeyan, "Detection of Leishmania infantum
kinetoplast DNA in the whole blood of asymptomatic individuals by PCR-ELISA and comparison with other infection markers in endemic areas, southern Iran," The American Journal of Tropical Medicine and Hygiene, vol.
Crithidia lucilia is a hemoflagellate protozoa that has an organelle called a
kinetoplast which contains a high-concentration native double-stranded DNA.
After 1 h at room temperature, we rinsed the slides in PBS, stained the nuclear and
kinetoplast DNA with DAPI, and mounted them with Vecta-Shield medium (Vector Laboratories).
Luteolin, an abundant dietary component is a potent anti-leishmanial agent that acts by inducing topoisomerase II mediated
kinetoplast DNA cleavage leading to apoptosis.
(nucleus,
kinetoplast, and cytoplasmic limit) was observed.
Further insight into the role of Top II-induced DSBs in mitochondria maybe drawn from parallels in Kinetoplastids, a group of flagellated protozoa which includes the parasitic Trypanosoma and Leishmania species, which possess a characteristic dense granule of DNA called a
kinetoplast within their single mitochondrion.