Molisch test

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Noun1.Molisch test - biochemical indicator of the presence of carbohydrates in a solution; if carbohydrates are present a violet ring is formed by reaction with alpha-naphthol in the presence of sulfuric acid
indicator - (chemistry) a substance that changes color to indicate the presence of some ion or substance; can be used to indicate the completion of a chemical reaction or (in medicine) to test for a particular reaction
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For each ethanol extract, presence or absence of sesquiterpenic lactones, coumarins, and cardiotonics were determined by ferric hydroxamate, Ehrlich method, fluorescence test, Baljet's test, and Molisch test. For the second phase of the phytochemical run, 25 mL ethanol extract from the first run was mixed with 25 mL petroleum ether.
Molisch test: Alpha naphthol and concentrated [H.sub.2]S[O.sub.4] were added to 2 mL solution of the extract.
After hydrolysis, the presence of carbohydrates in the hydrolyzed samples was investigated by different biochemical tests including Molisch Test, Iodine Test, Benedict's Test, Tollen Test, Barfoed's Test, Fehling's solution Test, Osazone Test, Seliwanoff's Test and Bial's Test.
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