The endothelium-dependent vasorelaxation was measured using the 620M
myograph system (DMT, Denmark) as described previously (9).
These ex vivo vascular rings were drawn by two stainless steel needles which were located at the bath of DMT
myograph (ADinstruments, Australia) to achieve 1 g tension and maintained for 1 h.
Isometric force generation was recorded with a Multi
Myograph System (Danish Myo Technology).
TA muscles were fastened with vicryl into the
myograph chambers (DMT, Denmark) and allowed to equilibrate under 15 mN (1.5 g) for 20 min, adjusting the tension periodically and replacing Krebs solution every 5 min.
Individual arterial ring segments of 2 mm length were mounted in a four channel small vessel wire
myograph (model 610M Danish Myotech, Denmark).
A segment (2 mm in length) of the mesentery was carefully cleared of adherent tissue and mounted in Krebs buffer at 37[degrees]C in a small vessel
myograph (model 400A, Danish Myotechnology, Denmark).
Fresh or incubated segments were immersed in a temperature-controlled (37[degrees]C)
myograph system (Organ Bath Model 700M0, J.P.
Detrusor strips were mounted on a
myograph system (Danish Myo Technology Model 800MS, Denmark) with carbogen-aerated Krebs' solution at 37[degrees]C.
Organ chambers (Multi
Myograph System, Danish Myo Technology A/S) were filled with (37[degrees]C) Krebs solution containing (in mmol/L) 119.0 NaCl, 4.7 KCl, 2.5 Ca[Cl.sub.2], 1.0 Mg[Cl.sub.2], 25.0 NaHC[O.sub.3], 1.2 K[H.sub.2]P[O.sub.4], and 11.0 D-glucose.
When the number of arterial segments was more than one in an individual, the average was used for that individual in
myograph experiment.