The NLRP3 inflammasome is a multiprotein complex composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC; also known as
PYCARD), the effector cysteine protease 1 (caspase 1), and NLRP3.
Deeg, "Stroma-dependent apoptosis in clonal hematopoietic precursors correlates with expression of
PYCARD," Blood, vol.
Remarkably, microarray analysis after LincRNA-EPS overexpression highlighted the repression of many proapoptotic genes, such as Bad, Bax, Caspase-2 and-6, Fadd,
Pycard, and with
Pycard being the most affected.
DNA Methylation and Patient Outcome.--DNA methylation status of individual genes, such as those encoding a transcriptional repressor (HIC1), a proapoptotic protein (
PYCARD), a tumor suppressor (LZTS1), and a transporter (SLC6A20), has been associated with either a good or poor prognosis.
After splitting the SSB-bound DNA into 10 different tubes, we still had sufficient material for successful MSP analysis of the CDKN2A, CDKN2B [cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)], and
PYCARD (PYD and CARD domain containing; also known as ASC and TMS1) promoters (data not shown).