Eleven different sugars including lactose, mannose, cellobiose,
raffinose, dextrose, arabinose, maltose, fructose, sucrose, xylose, and mannitol were used.
FODMAPs include fructose, lactose, sorbitol, fructans (e.g., fructooligosaccharides and inulin), and galactans (also called galactooligosaccharides; e.g.,
raffinose).
It happens due to the presence of a sugar called
raffinose, which the human body lacks the ability to break down.
Subsequently, 2mM
raffinose, as an internal standard used for normalization between samples, was added, and all samples were lyophilized and dissolved in methanol:water (1:1 v/v).
gallinarum was identified with ability to ferment mannitol,
raffinose.
Moreover, they fermented glucose, fructose, mannose, mannitol, sucrose, sorbitol and xylose without gas production but not ferment arabinose, inositol, lactose, maltose, salicin, dulcitol and
raffinose.
viswanathii assimilated sucrose and cellobiose but failed to assimilate trehalose and
raffinose. C.
For chromatin immunoprecipitation, L40 yeast strains expressing LexA-Med6p derivatives (in pCL313) were grown in synthetic media containing glucose (Glc) or galactose plus
raffinose (Gal + Raf) for 6h in the presence of 0.5 mM CuS[O.sub.4].
Lactose,
raffinose, and trehalose fermentation was assessed, using [S4074.sup.T] and [NM305.sup.T] as control strains, in PPLO broth (Difco) supplemented with 40 [micro]g/ml NAD, as described previously [1, 8].
It also contains many additional components such as lactobionate (100mmol/l),
raffinose (30mmol/l), adenosine (5mmol/l), allopurinol (1mmol/l), and glutathione (3mmol/l).
NPMPs of
raffinose pentahydrate and trehalose dihydrate were fabricated by typical co-SD approach in presence of a cyclic oligosaccharide (HP-[beta]-CD) to enhance its dispersion.
It is noteworthy that metabolic changes involved in the protection of seed tissues against desiccation damage occur during maturation, such as increased ABA (acid abscisic) content, LEA (late embryogenesis abundant) protein translation, increased sugar abundance such as sucrose,
raffinose and stachyose [30].