Determination of Contents of Carbohydrate,
Uronic Acid, and Protein.
The sugar monomer and
uronic acid content of the purified polysaccharides was influenced by both the type of fruit and fraction.
During the process, protopectin and pectin are degraded into galacturonic acid, increasing thus the content of
uronic acid and methanol as well as the total acidity of the product.
The exopolysaccharides produced from R.glutinins was a novel
uronic acid, mannose-rich acidic heteropolysaccharide composed of neutral sugars (85%)and
uronic acid (15%).
Pettersson, "Total dietary fiber determined as neutral sugar residues,
uronic acid residues, and Klason lignin (the Uppsala method): collaborative study," Journal of AOAC International, vol.
GAGs are linear polysaccharides made up of hundreds of repeating disaccharide units, which are composed of an amino sugar (N-acetylglucosamine, GlcNAc, N-acetylgalactosamine, GalNAc or glucosamine) and
uronic acid (glucuronic acid, GlcUA or Liduronic acid, IdoUA) or galactose (Gal, only keratan sulfate).
Method for
uronic acid microanalysis by normal-phase partition chromatography (NPPC) with postcolumn fluoresceence deriva- teization has been reported[18].
Compare to mango, ambarella pulp exhibits high
uronic acid (21 mg/g) content and low pH (3.9) [21]; meaning that preparation of ambarella jam will need less pectin than that of mango.
The total carbohydrate and
uronic acid contents were determined by the phenol-sulfuric acid (Dubois et al.
For glycosaminoglycan (GAG),
uronic acid and sialic acid analysis, 50 mg of velvet antler sample was decalcified in 1 ml 0.05 M [Na.sub.2]EDTA (pH 7.4, including 0.5 M Tris) for 2 days at 4[degrees]C.
Cell wall polysaccharides were quantified by the Uppsala total dietary fiber method for neutral monosaccharides and the Ahmed and Labavitch (1977) method for
uronic acid quantitation.