And chewing a conker - or its shell - can release a potentially fatal toxin called
aesculin which is found in all parts of the horse chestnut tree.
An aliquot of the broth was plated onto blood agar plates and incubated in a C[O.sub.2] incubator at 35[degrees]C overnight, and a bile
aesculin agar was inoculated.
Bu konuda yapilan calismalarda at kestanesi ozunde yer alan aescinin suda cozunurluk ve erime noktasina gore birbirinden ayirt edilen alfa ve beta olmak uzere 2 form triterpene saponin karisimindan olustugu; bunun disindaki bilesenlerin ise bioflavonoidler (quercetinve kaempferol),proanthocyanidin A2 (bir antioksidan) ve coumarinler (fraxin ve
aesculin) oldugu gosterilmistir.
colonies were confirmed in kanamycin
aesculin azide agar (Liofilchem, Roseto degli Abruzzi, Italy), incubated at 44[degrees]C for 4 h.
Zhang, "Micelle-mediated extraction and cloud point preconcentration for the analysis of
aesculin and aesculetin in Cortex fraxini by HPLC," Journal of Pharmaceutical and Biomedical Analysis, vol.
Arginine dihydrolase agar and
Aesculin Azide agar (Merck, Germany) were employed to perform the hydrolysis tests.
Samples were plated onto Sheep blood agar (5%>), MacConkey agar and Bile
Aesculin agar.
Catalase-negative organisms were tested on both bile
aesculin plates (Diagnostic Media Products, SA) and with the PYR 50 test (Remel, USA) to differentiate between Streptococci and Enterococci.
For secondary identification of the isolates to the species level, different biochemical tests (oxidation fermentation test, Mannitol and salicin sugar fermentation,
aesculin hydrolysis, and CAMP test) were done based on the genus ofbacteria to be identified [10].