The following effects of pretreatment are extremely important for the preparation of the optimal MLV precursor: breakdown of ester bonds between lignin and other constituents of the cross-linked lignocellulosic structure, partial destruction of the lignin matrix to smaller fragments, decrease in the polymerization degree of hemicellulose, reduction in the proportion of crystalline cellulosic form in favor of microfibril
amorphism, causal swelling of the lignocellulosic skeleton, and extraction of released inorganic matter from biomass.