Studies conducted on rodents, cattle and sheep have also shown the resulting decrease in number of healthy follicles and increase in atretic follicles.
Damietta showed the most degenerated features in the hermaphrodite gland as atretic oocytes and sperms and atrophy of most gland components (Figure 6h,6i).
observed apparent damage of the ovary with follicular cells dispersion and vacuolar epithelial cells on microscopic examination with increase in number of atretic follicles after lead exposure.19 The study also observed that lead causes reduction in the number of primary follicles and decrease the number of follicles that enter the growing phase.
Sections cut from paraffin-embedded ovaries were stained with hematoxylin and cosin to count preovulatory follicles, corpora lutea, antral follicles, and atretic follicles using a light microscope as described by Parborell et al.
A follicular wave is characterized by the emergence of a cohort of follicles [greater than or equal to] 4 mm in diameter (Knopf et al., 1989), from which one large follicle develops and subordinate follicles become atretic (Ginther et al., 1989).
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