Thus, at least some HCC tumors may be
auxotrophic for arginine and require arginine supply from extracellular sources (110).
Interestingly, the reversal of argininosuccinate lyase activity makes these cells
auxotrophic for arginine, which can have therapeutic applications for FH-deficient cancers (35).
Therefore, the
auxotrophic mutants which are unable to multiply in the environment were designed.
Of the 12 isolates, 7 were double
auxotrophic mutants with G6P and G3P (mean MIC 384 mg/L), 3 were
auxotrophic only for G3P (mean MIC 597 mg/L), and 2 were capable of using both substrates and exhibited high-level resistance (MIC >1,024 mg/L).
Auxotrophic selectable markers involved in amino acid or nucleotide biosynthesis have also been used.
More congruent nichespecific phenotypes were observed in isoleucine/valine
auxotrophic A.
anomalus as a platform to produce recombinant proteins such as the design of specific expression vectors and the generation of
auxotrophic strains.
(1977) The isolation and preliminary characterisation of
auxotrophic and analogue resistant mutants of the moss, Physcomitrella patens.
The colonies which showed growth on complete medium (containing all the essential amino acids) but not on minimal medium were considered as
auxotrophic (mutants).The mutants were then screened for hyper- production of alginate against the parent strain by using the pre-optimized conditions for wild culture.