Peroxidase activities were measured with 3,3',5,5'-tetra methyl
benzidine (TMB) as the reductive substrate (JOHNSEN & JACOBSEN, 2008).
After these procedures, color reaction with tetramethyl
benzidine was quantified in a plate reader at 450 nm (Quick Elisa, Thermo Fisher Scientific, USA).
Mutagenicity of
benzidine and benzidine-congener dyes and selected monoazo dyes in a modified Salmonella assay.
After, buffer washing, 100 ul of Tetra Methyl
Benzidine (TMB) substrate was added and incubated for 15 minutes at room temperature.
Slides were incubated in Diamino
benzidine (DAB) or other suitable peroxidase substrate.
Peroxidase (PER) activity was detected by spectrophotometry at 600 nm using
benzidine reagent (Litvin, 1981).
Exposure to certain industrial chemicals, such as aniline dyes,
benzidine and xenylamine, is also thought to be linked to a higher rates of bladder cancer.
Peroxidase activity was revealed by adding 100 [micro]l per well of tetra methyl
benzidine (Sigma-Aldrich) in dark.
The slides were developed using a diamino-
benzidine (DAB)-peroxidase substrate for 20-30 min at room temperature.
Controlling the FDR at 0.1, notable positive associations were for 1,4-dioxane, bromoform, dibenzofurans, glycol ethers, methyl ferf-butyl ether (MTBE), and propionaldehyde; whereas 1,4- dichlorobenzene, 4,4'-methylene diphenyl diisocyanate (MDI),
benzidine, and ethyl carbamate (urethane) were inversely associated with an ASD diagnosis.