D, Expression levels of proliferation-associated proteins were evaluated by western
blot analysis. E, Percentage of apoptotic cells was determined by FITC Annexin V/Dead Cell Apoptosis kit and flow cytometry.
Light chain (LC) II, LC3 I, and p62, p38 mitogen-activated protein kinase (MAPK) and their protein of phosphorylation of p38 (p-p38) were detected with Western
blot analysis. The protein levels of p-p53 (ser15) and their distribution were detected by immunofluorescence (IF).
The MW (~23 kDa) of IFN[alpha]2-T[alpha]1 was determined by 12 % SDS-PAGE and its integrity was confirmed by Immuno
blot analysis using anti-interferon [alpha]-2 and anti-thymosin [alpha]-1 antibodies.
We embedded brain and lymph node samples in paraffin wax, sectioned at 5 [micro]m, and stained with hematoxylin and eosin or subjected to immunohistochemical or paraffin-embedded tissue
blot analysis. We pretreated sections for immunohistochemistry with 98% formic acid for 5 min, followed by autoclaving in citrate buffer for 5 min at 121 [degrees]C.
Lung tissues were collected for histopathological staining, immunohistochemistry assessment, Western
blot analysis, and quantitative real-time PCR (qRT-PCR) examination.
Western
Blot Analysis. Rats were sacrificed by decapitation after AIM scoring on treatment Day 15.
Immunoprecipitation and Western
Blot Analysis. Cells, treated or not with 20 ng/ml KGF for the indicated times, were lysed in RIPA buffer.
(a) Representative and statistical results of Western
blot analysis showed the levels of cPKC[beta]II in the cortex, hippocampus, and striatum at 2 weeks after STZ injection.
(Guangzhou, China) for mass spectrometry (MS) detection and bioinformatic analysis, and the other was for Western
blot analysis.
Following assays were performed 48 h after transfection, including assessment of cell proliferation, cell cycle, apoptosis, migration, and western
blot analysis.
Western
Blot Analysis. Whole cell lysates were collected by adding Triton X-100 buffer (Roche Diagnostics).