In human injury response, c-Fos is paired with the gene
c-Jun. In axolotls, however, Echeverri and her team determined that c-Fos is activated with the gene JunB.
The membranes were incubated with primary antibodies, including GnRHR,
c-Jun, Elk-1, Egr-1, Nur77, CREB, PKC, ERK1/2, p38MAPK, JNK1/2, PKA, CAM, and tubulin (Abcam Ltd., USA) overnight at 4 AdegC.
The membranes were incubated with primary antibodies against cyclinD1 (ab134175), p21 (ab109199), cytochrome C (ab90529), B cell lymphoma-2 (Bcl-2, ab196495), Bcl-2-associated X protein (Bax, ab182733), cleaved caspase-3 (ab2302), cleaved caspase-9 (ab2324), matrix metalloproteinase (MMP)-2 (ab97779), MMP-9 (ab137867), vimentin (ab137321),
c-Jun (ab32137), phospho (p)-c-Jun (ab32385), [beta]-actin (ab8229, all Abcam, Cambridge, UK),
c-Jun N-terminal protein kinase (JNK; 9252), or p-JNK (9251, both Cell Signaling Technology, USA) at 4[degrees]C overnight.
Quantitative real-time (qrt)-PCR was used to determine the mRNA expression of Wnt5a, PTEN, CTNNB1, MAG, CCND1,
c-Jun , and glyceraldehyde-3-phosphate dehydrogenase.
Tres mecanismos han mostrado ser relevantes en el humano: induccion del estres oxidativo, dano del ADN mitocondrial y la activacion del factor de transcripcion JNK (cinasas
c-jun N-terminal) (22).
The Figure 7 showed serum protein levels of transcription factors
c-JUN predominantly in WD exposed rats in both 5 days and 10 days exposure group.
In a study published in the journal Nature Communications, researchers uncovered a molecular "switch" that occurs when a protein that helps to drive the body's response to exercise, called
c-Jun N-terminal kinase (JNK), is activated.
MG132 (proteasome inhibitor) and SP600125 (
c-Jun N-terminal kinase inhibitor, JNK inhibitor) were purchased from Calbiochem (La Jolla, CA).
AM-111, which is being evaluated in two Phase 3 clinical trials, contains the synthetic peptide brimapitide (also called D-JNKI-1, D-stereoisomer of
c-Jun N-terminal Kinase Inhibitor 1), a cell-penetrating inhibitor of the JNK stress kinase.